For general feedback, use the public comments section below (please adhere to guidelines). Associate Professor doi: 10.1056/NEJMoa2027906. YDM: One of the limitations of our study is that we dont know if what we observed in this cell line could also happen in cells of other tissue types, and this needs to be addressed in follow-up studies. The mRNA then leaves the nucleus and enters the cells cytoplasm, where it translates into LINE-1 protein. The authors of thefirst reported caseof a healthy 35-year-old female who developed autoimmune hepatitis a week after her first dose of the Pfizer COVID-19 vaccine said that there is a possibility that spike-directed antibodies induced by vaccination may also trigger autoimmune conditions in predisposed individuals as it has been shown that severe cases of SARS-CoV-2 infection are characterized by an autoinflammatory dysregulation that contributes to tissue damage, which the viruss spike protein appears to be responsible for. (In-vitro Swedish Study) https://youtube.com/live/MjxlvduyJyc?feature=share 28 Feb 2023 02:39:58 The Food and Drug Administration has flagged Guillain-Barre syndrome, a rare neurological condition, as a potential risk for Pfizers respiratory syncytial virus vaccine. In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage The persistence of the spike protein in the body raises the prospect of sustained inflammation within and damage to organs which express the spike protein, according to experts atDoctors for COVID Ethics,an organization consisting of physicians and scientists seeking to uphold medical ethics, patient safety, and human rights in response to COVID-19.. A gene that the researchers studied produces the protein LINE-1. and transmitted securely. The pharmaceutical industry is dangerous to health. "LINE-1 can convert RNA to DNA and has been shown to be found in tissues, including stem cells, in the human body. eCollection 2022 Dec. Mavridis C, Aidonidis G, Evangelou M, Kalogeridis A. Hist Philos Life Sci. 2022 The Childrens Hospital of Philadelphia. The results have been widely discussed across social media but the results have in many cases been misinterpreted. The content provided in this video is for informational and educational purposes only.\rPlease consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. In the cell line in a petri dish, you dont have those checks in place.. The study, conducted by researchers at Lund University in Sweden and published in a peer-reviewed journal, investigated the effect of Pfizers mRNA COVID-19 vaccine on a human cell line that was derived from liver cancer tissue. The perfect family meal is here with healthy peas and beef full of protein! Markus Aldn et al, Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line, Current Issues in Molecular Biology (2022). These tools for evaluating information may be useful: Categories: In the general population, the antibody response wanes over the course of time after two doses of the vaccination, but seems to be more Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. Thank you for taking time to provide your feedback to the editors. YDM: This study does not investigate whether the Pfizer vaccine alters our genome. The researchers write that the findings warrant a further The whole process of Pfizers mRNA transfecting liver cells occurred within just six hours, per the study. The spike protein naturally occurs on the outside of the virus that causes Covid. Professor at Infection Medicine badbunny. Montoya JG, Adams AE, Bonetti V, Deng S, Link NA, Pertsch S, Olson K, Li M, Dillon EC, Frosch DL. Missing context. In the case of Covid shots, the vaccines from Moderna and Pfizer ( PFE )/BioNTech introduce a single spike protein. doi: 10.1038/s41586-020-2639-4. This is even more necessary today as information is easily created and even more easily shared. RNA was purified and qPCR was performed using primers targeting BNT162b2. Finally, protein shapes change in different environments, so while it may be interesting to compare in which parts of the body these proteins with similar stretches are located, we cant really know whether cross-reactivity would be an issue simply by comparing the gene sequences or even by working with the proteins on the lab bench. Imaging findings included inflammation, beading, stricturing, and dilation of the biliary tree. Pathogenic priming likely contributes to serious and critical illness and mortality in COVID-19 via autoimmunity. Your browser has javascript turned off or blocked. Microbiol Spectr. Moore also cautioned that the cells used in the study were quite different than primary cells in the body. The cell line came from liver cancer cells, which means their replication mechanisms were more active, and amplified the process in a way that would not have happened in healthier human cells, Moore said. RNA is closely related to DNA, and one type, called messenger RNA or mRNA, sends instructions to the cell for different purposes. 2020 Apr 9;3:100051. However, this is not an appropriate conclusion from this paper for several reasons: Logical fallacy called hasty generalization (It is important to note in this case, that the misconception was not because of the quality of the science or the messaging of the authors, but rather because others took the findings out of context.). BNT: BNT162b2; L: DNA ladder; Ctrl1: cultured Huh7 cells; Ctrl2: Huh7 cells without BNT162b2 treatment collected at 6 h; Ctrl3: Huh7 cells without BNT162b2 treatment collected at 24 h; Ctrl4: Huh7 cells without BNT162b2 treatment collected at 48 h; Ctrl5: RNA from Huh7 cells treated with 0.5 g/mL of BNT162b2 for 6 h; Ctrl6: RNA from Huh7 cells treated with 0.5 g/mL of BNT162b2 for 6 h, digested with RNase. Accessibility part may be reproduced without the written permission. :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rChapters00:00 introduction00:45 Link to the summary01:00 Review of the reference material02:19 Cell preparation for the vaccine03:35 Vaccine addition to the cells06:00 RT-PCR for find RNA and then DNA13:39 Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA18:27 Measuring the volume of proteins in the cytosol and the nucleus22:35 DNA purification and verification28:41 Results28:42 LNP enters the liver cells efficiently29:33 LINE-1 gene is expressed30:46 ORF-1 found in cytosol and the nucleus36:01 DNA from the vaccine found in the cell43:30 Questions not answeredDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. By using our site, you acknowledge that you have read and understand our Privacy Policy DNA amplicons (444 bps) were visualized on agarose gel. Bad Bunny. Please select the most appropriate category to facilitate processing of your request. Telephone: +46 (0)46 222 00 00 (switchboard) Differences in mRNA-1273 (Moderna) and BNT162b2 (Pfizer-BioNTech) SARS-CoV-2 vaccine immunogenicity among patients undergoing dialysis. The results have been widely discussed across social mediabut the results have in many cases been misinterpreted. Let's review the details of their method.\r\rIf you like this content and want more, I am doing a special lifetime membership offer. Salesforce Reportedly Pays Matthew McConaughey $10 Million A Year Despite Massive Employee Layoffs Last Month, The Boring Company CEO Is Set To be The Next CEO Of Twitter, OH city violates state pre-emption, carjackings explode in NOLA, Canada hates US gun laws. See this image and copyright information in PMC. Huh7 cells were treated without (Ctrl) or with 0.5 g/mL of BNT162b2 for 6, 24, and 48 h. Genomic DNA was purified and digested with 100 g/mL RNase. A gene that the researchers studied produces the protein LINE-1. The study tested whether the vaccines selenagomez. While the Moderna vaccines are meeting this specification, the Pfizer vaccines are 10 fold higher in contamination with 1 DNA molecule per 350 mRNAs. By Meiling Lee. This, of course, does not mean these data are not important. Intracellular reverse transcription of Pfizer BioNTech COVID-19 mRNA vaccine BNT162b2 in vitro in human liver. DETAILS - Pfizer Vaccine Becomes DNA in The Human Liver Cells Huh7. -, Walsh E.E., Frenck R.W., Jr., Falsey A.R., Kitchin N., Absalon J., Gurtman A., Lockhart S., Neuzil K., Mulligan M.J., Bailey R., et al. Philadelphia, PA 19104, Know My Rights About Surprise Medical Bills. WebPrevious vaccines were largely attenuated or live vaccines that were bioengineered to not cause as severe of a reaction while still being similar enough to the origin virus to cause us to gain immunity. As such, even if DNA representing the viral RNA was integrated into the cell, no other cells with the altered DNA would be produced. Genomics, diabetes and endocrinologyyang [dot] de_marinis [at] med [dot] lu [dot] se, Box 117, 221 00 Lund, Sweden WebThe interaction between pre-existing liver disease and coronavirus infection has been illustrated, whereby coronaviruses influence the occurrence, severity, prognosis and treatment of liver diseases. Our publication is the first in vitro study on the conversion of mRNA vaccine into DNA, inside cells of human origin. BNT162b2 is another name for the Pfizer-BioNTech COVID-19 vaccine that is marketed under the brand name Comirnaty. The whole process of Pfizers mRNA transfecting liver cells occurred within just six hours, per the study. Lund University This would be like saying that two people have the same social security number because both contain the number 5. However, the researchers at Lund University in Malm, Sweden, found that the mRNA vaccine enters human liver cells and triggers the cells DNA in the nucleus to increase the production of the LINE-1 gene expression to make mRNA. followers 26 videos. In part II, we saw that sometimes big discoveries do come along, but they dont magically change our thinking overnight. (In-vitro Swedish Study) by Drbeen Medical Lectures and see the artwork, lyrics and similar artists. Sudden deaths of Canadian WebSkip to topic navigation. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. Science X Daily and the Weekly Email Newsletter are free features that allow you to receive your favorite sci-tech news updates in your email inbox, New HIV mRNA vaccine study gets boost from COVID mRNA vaccine development, How gut bacteria can impact treatments for cancer, Human feces and urine contain a motherlode of health data: 'Smart toilets' detect daily fluctuations, serious disease, Multisite multiomic analysis reveals diverse resistance mechanisms in end-stage ovarian cancer, Review of data surrounding COVID vaccines and pregnant women suggest no increased risk of miscarriage, A possible way to prevent or treat lung damage associated with long COVID. (In-vitro Swedish Study)\r\rAn accepted and published Swedish study demonstrates for the first time the presence of Pfizer-BionTech vaccine DNA in the cell. Said another way, it was an . Materials in this section are updated as new information and vaccines become available. 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pfizer vaccine becomes dna in liver